A vibration is a factor that impairs an working environment of agricultural tractor drivers and threatens their health. This study focused on the evaluation of whole-body vibration for the agricultural tractor driver according to an international standard (ISO 2631-1). Vibration tests were performed by applying six road conditions on a four-post road simulator that guarantees reproducibility and repeatability. The vibration characteristics were analyzed using the crest factor, maximum transient vibration value, vibration dose value, and weighted root-mean-square acceleration from the obtained vibration signals. Finally, the whole body vibration of the agricultural tractor driver were quantitatively evaluated by applying the health guidance caution level, and the comfort reactions for vibration environment to the root-mean-square acceleration and the vibration dose value according to the evaluation methods.