Agricultural tractors comprise power take-off (PTO) drivelines, which that transmits engine power to the outside for various agricultural work, and various mechanical components are connected by spline couplings with tolerances. Dynamic behavior of the spline couplings causes a jumping phenomenon where the rotation speed fluctuation amplifies and then rapidly decreases. This paper experimentally examined the effects of spline tolerance on the dynamic behavior and PTO gear rattle noise for four different spline tolerance levels. For the fit tolerance (0.1 mm), no jumping was observed. For increasing tolerance levels of 0.5, 0.9, and 1.3 mm, the jumping rotation speed decreased to 920, 890, and 880 rpm, respectively. With increasing tolerance, both the rotation speed fluctuation and noise level immediately before jumping increased gradually. Furthermore, for the specimen with tolerance levels of 1.3 mm(type D), a transient range was observed in which the dynamic behaviors before and after jumping were mixed and rotation speed fluctuation and sound pressure level showed large deviations from their respective average values.